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Title: Beta-oxidation in hepatocyte cultures from mice with peroxisomal gene knockouts
Authors: Dirkx, Ruud
Meyhi, Els
Asselberghs, Stanny
Reddy, Janardan
Baes, Myriam
Van Veldhoven, Paul P # ×
Issue Date: 29-May-2007
Series Title: Biochemical and Biophysical Research Communications vol:357 issue:3 pages:718-723
Abstract: Beta-oxidation of carboxylates takes place both in mitochondria and peroxisomes and in each pathway parallel enzymes exist for each conversion step. In order to better define the substrate specificities of these enzymes and in particular the elusive role of peroxisomal MFP-1, hepatocyte cultures from mice with peroxisomal gene knockouts were used to assess the consequences on substrate degradation. Hepatocytes from mice with liver selective elimination of peroxisomes displayed severely impaired oxidation of 2-methylhexadecanoic acid, the bile acid intermediate trihydroxycholestanoic acid (THCA), and tetradecanedioic acid. In contrast, mitochondrial beta-oxidation rates of palmitate were doubled, despite the severely affected inner mitochondrial membrane. As expected, beta-oxidation of the branched chain compounds 2-methylhexadecanoic acid and THCA was reduced in hepatocytes from mice with inactivation of MFP-2. More surprisingly, dicarboxylic fatty acid oxidation was impaired in MFP-1 but not in MFP-2 knockout hepatocytes, indicating that MFP-1 might play more than an obsolete role in peroxisomal beta-oxidation.
URI: 
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Cell Metabolism
Laboratory of Lipid Biochemistry and Protein Interactions
× corresponding author
# (joint) last author

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