Expression profiling of budding cells in colorectal cancer reveals an EMT-like phenotype and molecular subtype switching

De Smedt, Linde; Palmans, Sofie; Andel, Daan; Govaere, Olivier; Boeckx, Bram; Smeets, Dominiek; Galle, Eva; Wouters, Jasper; Barras, David; Suffiotti, Madeleine; Dekervel, Jeroen; Tousseyn, Thomas; De Hertogh, Gert; Prenen, Hans; Tejpar, Sabine; Lambrechts, Diether; Sagaert, Xavier

doi:10.1038/bjc.2016.382

Expression profiling of budding cells in colorectal cancer reveals an EMT-like phenotype and molecular subtype switching

Author:

De Smedt, Linde

Palmans, Sofie ; Andel, Daan ; Govaere, Olivier ; Boeckx, Bram ; Smeets, Dominiek ; Galle, Eva ; Wouters, Jasper ; Barras, David ; Suffiotti, Madeleine ; Dekervel, Jeroen ; Tousseyn, Thomas ; De Hertogh, Gert ; Prenen, Hans ; Tejpar, Sabine ; Lambrechts, Diether ; Sagaert, Xavier

Keywords:

Science & Technology, Life Sciences & Biomedicine, Oncology, colorectal cancer, budding, epithelial mesenchymal transition, gene expression profiling, EPITHELIAL-MESENCHYMAL TRANSITION, HIGH-RISK PATIENTS, PROGNOSTIC VALUE, TARGET GENES, I-CISTARGET, E-CADHERIN, PROGRESSION, ALIGNMENT, SURVIVAL, INVASION, Adult, Aged, Aged, 80 and over, Cell Division, Cell Proliferation, Colorectal Neoplasms, Epithelial-Mesenchymal Transition, Female, Gene Expression Profiling, Genes, Switch, Humans, Male, Margins of Excision, Middle Aged, Neoplasm Invasiveness, Phenotype, Tissue Array Analysis, Transcriptome, 1112 Oncology and Carcinogenesis, 1117 Public Health and Health Services, Oncology & Carcinogenesis, 3211 Oncology and carcinogenesis

Abstract:

Tumour budding, described as the presence of single cells or small clusters of up to five tumour cells at the invasive margin, is established as a prognostic marker in colorectal carcinoma. In the present study, we aimed to investigate the molecular signature of tumour budding cells and the corresponding tumour bulk.