Title: Positive feedback keeps duration of mitosis temporally insulated from upstream cell-cycle events
Authors: Araujo, Ana Rita
Gelens, Lendert
Sheriff, Rahuman
Santos, Silvia D.M. # ×
Issue Date: 20-Oct-2016
Publisher: Cell Press
Series Title: Molecular Cell vol:64 pages:362-375
Abstract: Cell division is characterized by a sequence of events by which a cell gives rise to two daughter cells. Quan- titative measurements of cell-cycle dynamics in sin- gle cells showed that despite variability in G1-, S-, and G2 phases, duration of mitosis is short and remarkably constant. Surprisingly, there is no corre- lation between cell-cycle length and mitotic duration, suggesting that mitosis is temporally insulated from variability in earlier cell-cycle phases. By combining live cell imaging and computational modeling, we showed that positive feedback is the molecular mechanism underlying the temporal insulation of mitosis. Perturbing positive feedback gave rise to a sluggish, variable entry and progression through mitosis and uncoupled duration of mitosis from vari- ability in cell cycle length. We show that positive feedback is important to keep mitosis short, con- stant, and temporally insulated and anticipate it might be a commonly used regulatory strategy to create modularity in other biological systems.
ISSN: 1097-2765
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Dynamics in Biological Systems
× corresponding author
# (joint) last author

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