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Title: Application of a dynamic metabolic flux algorithm during a temperature-induced lag phase
Authors: Vercammen, Dominique
Telen, Dries
Nimmegeers, Philippe
Janssens, Arno
Akkermans, Simen
Noriega Fernandez, Estefania
Logist, Filip
Van Impe, Jan # ×
Issue Date: Mar-2017
Publisher: The Institution
Series Title: Food and Bioproducts Processing vol:102 issue:Epub ahead of print pages:1-19
Abstract: In predictive microbiology, the (induced) lag-phase is a phenomenon of specific interest, as it has a large impact on the assessment of safety and quality of food products. This lag phase has been studied mostly on a macroscopic level. However, a quest for more mechanistically-based predictive models has started, for example, through the integration of a metabolic reaction network into widely used macroscopic model structures. This multi-scale modeling approach is called dynamic metabolic flux analysis (dMFA). In this contribution, a recently developed algorithm for dMFA is used to estimate the metabolic fluxes in Escherichia coli K12 during an experimentally induced lag phase through a sudden shift in temperature. To study this phenomenon, controlled bioreactor experiments were performed: on the one hand at a fixed and optimal temperature for growth (37 °C), and on the other hand starting at 20 °C, with a sudden temperature shift to 37 °C during the exponential growth, inducing an intermediate lag phase. The evolution of biomass and metabolite concentrations was monitored during these experiments. After dMFA analysis of the gathered measurements, some interesting patterns in metabolic activity during the different growth phases are revealed. The described case study is a first practical test case to assess the capabilities of the recently developed dMFA methodology in an experimental predictive microbiology setting.
ISSN: 0960-3085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Sustainable Chemical Process Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
UC Limburg - miscellaneous
Sustainable Chemical Process Technology TC
Bio- & Chemical Systems Technology, Reactor Engineering and Safety Section
Sustainable Chemical Process Technology TC, Technology Campuses Ghent and Aalst
× corresponding author
# (joint) last author

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