PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay® in 201 patients with haematological disease suspected for invasive aspergillosis
Chong, G M × van der Beek, M T von dem Borne, P A Boelens, J Steel, E Kampinga, G A Span, L F R Lagrou, Katrien Maertens, J A Dingemans, G J H Gaajetaan, G R van Tegelen, D W E Cornelissen, J J Vonk, A G Rijnders, B J A #
Oxford University Press
The Journal of Antimicrobial Chemotherapy vol:Ahead of print
In patients with invasive aspergillosis (IA), fungal cultures are mostly negative. Consequently, azole resistance often remains undetected. The AsperGenius(®) multiplex real-time PCR assay identifies clinically relevant Aspergillus species and four resistance-associated mutations (RAMs; TR34/L98H/T289A/Y121F) in the Cyp51A gene. This multicentre study evaluated the diagnostic performance of this assay on bronchoalveolar lavage (BAL) fluid and correlated the presence of RAMs with azole treatment failure and mortality.