Title: Detection of natural resistance-associated substitutions by ion semiconductor technology in HCV1b positive, direct-acting antiviral agents-naïve patients
Authors: Marascio, Nadia ×
Pavia, Grazia
Strazzulla, Alessio
Dierckx, Tim
Cuypers, Lize
Vrancken, Bram
Barreca, Giorgio Settimo
Mirante, Teresa
Malanga, Donatella
Oliveira, Duarte Mendes
Vandamme, Anne-Mieke
Torti, Carlo
Liberto, Maria Carla
Focà, Alfredo #
Issue Date: 27-Aug-2016
Publisher: MDPI
Series Title: International Journal of Molecular Sciences vol:17 issue:9
Article number: 1416
Abstract: Naturally occurring resistance-associated substitutions (RASs) can negatively impact the response to direct-acting antivirals (DAAs) agents-based therapies for hepatitis C virus (HCV) infection. Herein, we set out to characterize the RASs in the HCV1b genome from serum samples of DAA-naïve patients in the context of the SINERGIE (South Italian Network for Rational Guidelines and International Epidemiology, 2014) project. We deep-sequenced the NS3/4A protease region of the viral population using the Ion Torrent Personal Genome Machine, and patient-specific majority rule consensus sequence summaries were constructed with a combination of freely available next generation sequencing data analysis software. We detected NS3/4A protease major and minor variants associated with resistance to boceprevir (V36L), telaprevir (V36L, I132V), simeprevir (V36L), and grazoprevir (V36L, V170I). Furthermore, we sequenced part of HCV NS5B polymerase using Sanger-sequencing and detected a natural RAS for dasabuvir (C316N). This mutation could be important for treatment strategies in cases of previous therapy failure.
ISSN: 1422-0067
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
ijms-17-01416.pdf Published 1208KbAdobe PDFView/Open Request a copy
2016174.pdfOA article Published 728KbAdobe PDFView/Open

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science