WG2 TRAINING SCHOOL on Interactions in Complex Disease Analysis location:Antwerpen date:26-29 April 2016
By translating the CUG codon into serine rather than leucine Candida albicans does not follow the universal genetic code. This makes it hard to use the model system, Saccharomyces cerevisiae, as a host for two-hybrid experiments. To overcome this problem we have developed a candida two hybrid (C2H) system to screen for protein-protein interactions in C. albicans. With the availability of the orfeome collection (containing all the ORFs from C. albicans in gateway adapted vectors) is it now also possible to apply a high-throughput approach using the C2H system. In order to set up this high-throughput C2H system the two hybrid vectors had to be made gateway compatible and a mating strategy had to be optimized and tested.
By applying the gateway system, the mating strategy and using approximately 12000 potential protein-protein interactions to test the system we now have an optimized a high-throughput C2H system.