Title: A high-throughput Candida albicans two hybrid (C2H) system
Authors: Schoeters, Floris
Van Dijck, Patrick
Issue Date: 28-Apr-2016
Conference: WG2 TRAINING SCHOOL on Interactions in Complex Disease Analysis location:Antwerpen date:26-29 April 2016
Abstract: By translating the CUG codon into serine rather than leucine Candida albicans does not follow the universal genetic code. This makes it hard to use the model system, Saccharomyces cerevisiae, as a host for two-hybrid experiments. To overcome this problem we have developed a candida two hybrid (C2H) system to screen for protein-protein interactions in C. albicans. With the availability of the orfeome collection (containing all the ORFs from C. albicans in gateway adapted vectors) is it now also possible to apply a high-throughput approach using the C2H system. In order to set up this high-throughput C2H system the two hybrid vectors had to be made gateway compatible and a mating strategy had to be optimized and tested.
By applying the gateway system, the mating strategy and using approximately 12000 potential protein-protein interactions to test the system we now have an optimized a high-throughput C2H system.
Publication status: published
KU Leuven publication type: AMa
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous

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