Title: Analyzing protein clusters on the plasma membrane: application of spatial statistical analyses on super-resolution microscopy images
Authors: Paparelli, Laura
Corthout, Nikky
Pavie, Benjamin
Annaert, Wim
Munck, Sebastian # ×
Issue Date: 2016
Publisher: Springer
Series Title: Advances in Anatomy, Embryology and Cell Biology vol:219 pages:95-122
Abstract: The spatial distribution of proteins within the cell affects their capability to interact with other molecules and directly influences cellular processes and signaling. At the plasma membrane, multiple factors drive protein compartmentalization into specialized functional domains, leading to the formation of clusters in which intermolecule interactions are facilitated. Therefore, quantifying protein
distributions is a necessity for understanding their regulation and function. The recent advent of super-resolution microscopy has opened up the possibility of
imaging protein distributions at the nanometer scale. In parallel, new spatial analysis methods have been developed to quantify distribution patterns in super-resolution
images. In this chapter, we provide an overview of super-resolution microscopy and summarize the factors influencing protein arrangements on the plasma membrane. Finally, we highlight methods for analyzing clusterization of plasma membrane proteins, including examples of their applications.
ISSN: 0301-5556
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory of Membrane Trafficking
× corresponding author
# (joint) last author

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