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Title: Investigation of the metabolic consequences of impregnating spinach leaves with trehalose and applying a pulsed electric field
Authors: Dymek, Katarzyna ×
Panerese, Valentina
Herremans, Els
Cantre, Dennis
Schoo, Rick
Toraño, Javier Sastre
Schluepmann, Henriette
Wadso, Lars
Verboven, Pieter
Nicolai, Bart
Dejmek, Petr
Galindo, Federico Gomez #
Issue Date: Dec-2016
Publisher: Elsevier
Series Title: Bioelectrochemistry vol:112 pages:153-157
Abstract: The impregnation of leafy vegetables with cryoprotectants using a combination of vacuum impregnation (VI) and pulsed electric fields (PEF) has been proposed by our research group as a method of improving their freezing tolerance
and consequently their general quality after thawing. In this study, we have investigated the metabolic consequences of the combination of these unit operations on spinach. The vacuum impregnated spinach leaves showed a drastic decrease in the porosity of the extracellular space. However, at maximum weight gain, randomly located air pockets remained, which may account for oxygen-consuming pathways in the cells being active after VI. The metabolic activity of the impregnated leaves showed a drastic increase that was further enhanced by the application of PEF to the impregnated tissue. Impregnating the leaves with trehalose by VI led to a significant accumulation of trehalose-6-phosphate (T6P), however, this was not further enhanced by PEF. It is suggested that the accumulation of T6P in the leaves may increase metabolic activity, and increase tissue
resistance to abiotic stress.
URI: 
ISSN: 1567-5394
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
× corresponding author
# (joint) last author

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