Title: Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function
Authors: Houbrechts, Anne ×
Vergauwen, Lucia
Bagci, Enise
Van houcke, Jolien
Heijlen, Marjolein
Kulemeka, Bernard
Hyde, David R
Knapen, Dries
Darras, Veerle #
Issue Date: Mar-2016
Publisher: Elsevier
Series Title: Molecular and Cellular Endocrinology vol:424 pages:81-93
Article number: S0303-7207(16)30018-1
Abstract: Retinal development in vertebrates relies extensively on thyroid hormones. Their local availability is tightly controlled by several regulators, including deiodinases (Ds). Here we used morpholino technology to explore the roles of Ds during eye development in zebrafish. Transcriptome analysis at 3 days post fertilization (dpf) revealed a pronounced effect of knockdown of both T4-activating Ds (D1D2MO) or knockdown of T3-inactivating D3 (D3bMO) on phototransduction and retinoid recycling. This was accompanied by morphological defects (studied from 1 to 7 dpf) including reduced eye size, disturbed retinal lamination and strong reduction in rods and all four cone types. Defects were more prominent and persistent in D3-deficient fish. Finally, D3-deficient zebrafish larvae had disrupted visual function at 4 dpf and were less sensitive to a light stimulus at 5 dpf. These data demonstrate the importance of TH-activating and -inactivating Ds for correct zebrafish eye development, and point to D3b as a central player.
ISSN: 0303-7207
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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