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Title: Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion
Authors: Quétier, Ivan ×
Marshall, Jacqueline J T
Spencer-Dene, Bradley
Lachmann, Sylvie
Casamassima, Adele
Franco, Claudio
Escuin, Sarah
Worrall, Joseph T
Baskaran, Priththivika
Rajeeve, Vinothini
Howell, Michael
Copp, Andrew J
Stamp, Gordon
Rosewell, Ian
Cutillas, Pedro
Gerhardt, Holger
Parker, Peter J
Cameron, Angus J M #
Issue Date: Jan-2016
Publisher: Elsevier Inc
Series Title: Cell Reports vol:14 issue:3 pages:440-8
Article number: 10.1016/j.celrep.2015.12.049
Abstract: In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including the Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse PKN isoforms and reveal that PKN2 loss results in lethality at embryonic day 10 (E10), with associated cardiovascular and morphogenetic defects. The cardiovascular phenotype was not recapitulated by conditional deletion of PKN2 in endothelial cells or the developing heart. In contrast, inducible systemic deletion of PKN2 after E7 provoked collapse of the embryonic mesoderm. Furthermore, mouse embryonic fibroblasts, which arise from the embryonic mesoderm, depend on PKN2 for proliferation and motility. These cellular defects are reflected in vivo as dependence on PKN2 for mesoderm proliferation and neural crest migration. We conclude that failure of the mesoderm to expand in the absence of PKN2 compromises cardiovascular integrity and development, resulting in lethality.
URI: 
ISSN: 2211-1247
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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