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Title: Single-Molecule Analyte Recognition with ClyA Nanopores Equipped with Internal Protein Adaptors
Authors: Biesemans, Annemie *
Soskine, Misha *
Maglia, Giovanni # ×
Issue Date: 14-Apr-2015
Publisher: American Chemical Society
Series Title: Journal of the American Chemical Society vol:137 issue:17 pages:5793-5797
Abstract: Nanopores have been used to detect molecules, to sequence DNA, or to investigate chemical reactions at the single-molecule level. Because they approach the absolute limit of sensor miniaturization, nanopores are amenable to parallelization and could be used in single-cell measurements. Here we show that single enzymes can be functionally and reversibly trapped inside the confined space of a ClyA nanopore. Remarkably, the binding of ligands to the internalized proteins is mirrored by specific changes to the nanopore conductance. Conveniently, the manipulation of the charge of the protein allowed increasing of the residence time of the
protein inside the nanopore. Nanopores with internalized protein adaptors can be used to study proteins in real time or can be incorporated into inexpensive portable devices for the detection of analytes with high selectivity.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
* (joint) first author
× corresponding author
# (joint) last author

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