A weakly acidic solution containing deoxycholic acid induces esophageal epithelial apoptosis and impairs integrity in an in vivo perfusion rabbit model

Pardon, Nicolas; Vicario, Maria; Vanheel, Hanne; Vanuytsel, Tim; Ceulemans, Laurens; Vieth, Michael; Jimenez, Marcel; Tack, Jan; Farré, Ricard

doi:10.1152/ajpgi.00370.2015

A weakly acidic solution containing deoxycholic acid induces esophageal epithelial apoptosis and impairs integrity in an in vivo perfusion rabbit model

Author:

Pardon, Nicolas

Vicario, Maria ; Vanheel, Hanne ; Vanuytsel, Tim ; Ceulemans, Laurens ; Vieth, Michael ; Jimenez, Marcel ; Tack, Jan ; Farré, Ricard

Keywords:

Science & Technology, Life Sciences & Biomedicine, Gastroenterology & Hepatology, Physiology, apoptosis, bile acid, GERD, permeability, weakly acidic reflux, GASTROESOPHAGEAL-REFLUX DISEASE, DILATED INTERCELLULAR SPACES, MUCOSAL INTEGRITY, GASTRIC-ACID, BILE-ACIDS, BASE-LINE, PATHOGENESIS, PERMEABILITY, RESISTANCE, HEARTBURN, Animals, Apoptosis, Deoxycholic Acid, Electric Impedance, Esophageal Mucosa, Extracellular Space, Gastroesophageal Reflux, Humans, Hydrogen-Ion Concentration, Male, Middle Aged, Models, Animal, Perfusion, Rabbits, Time Factors, 0606 Physiology, 1116 Medical Physiology, 3202 Clinical sciences, 3208 Medical physiology

Abstract:

Impaired esophageal mucosal integrity may be an important contributor in the pathophysiology of GERD. Nevertheless, the effect of potentially harmful agents on epithelial integrity is mainly evaluated in vitro, for a short period of time and the possible induction of epithelial apoptosis has been neglected.