Title: Dynamic simulation of in vitro micro-tissue formation using a particle-based deformable cell model
Authors: Smeets, Bart
Leijten, Jeroen
Odenthal, Tim
Teixeira, Liliana
Luyten, Frank
Van Oosterwyck, Hans
Ramon, Herman
Issue Date: 28-Sep-2015
Conference: International Conference on Particle-based Methods edition:4 location:Barcelona date:28-30 September 2015
Abstract: The three dimensional nature of our bodily tissues regulate auto- and paracrine signalling and establish natural biophysical conditions affecting both cell function and fate. Micro- tissues are excellently suited to study these important biological behaviours. By seeding cells on micro-wells of a non-adhesive substrate such as agarose, the cells effectively de-wet from the surface and become spherical three-dimensional micro-tissues. First, the cells actively migrate towards each other to form a loose cluster of cells. Next, the clusters condensate into smooth, spherical micro-aggregates. In this study, we use particle-based models to simulate the dynamics of aggregate formation and to investigate the most important parameters affecting the final aggregate shape, cell morphology, mechanical stress, and the dynamics of its formation (see Figure 1). We construct a phase-diagram of this system, which predicts de-wetting as a function of cell contractility, cell-substrate adhesion and cell motility.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Mechanical Engineering - miscellaneous
Skeletal Biology and Engineering Research Center (+)
Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Biomechanics Section

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