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Title: The Influence of Dietary Protein Intake on Mammalian Tryptophan and Phenolic Metabolites
Authors: Poesen, Ruben ×
Mutsaers, Henricus A M
Windey, Karen
van den Broek, Petra H
Verweij, Vivienne
Augustijns, Patrick
Kuypers, Dirk
Jansen, Jitske
Evenepoel, Pieter
Verbeke, Kristin
Meijers, Björn
Masereeuw, Rosalinde #
Issue Date: 2015
Publisher: Public Library of Sciene
Series Title: PLoS One vol:10 issue:10 pages:e0140820
Article number: 10.1371/journal.pone.0140820
Abstract: Although there has been increasing interest in the use of high protein diets, little is known about dietary protein related changes in the mammalian metabolome. We investigated the influence of protein intake on selected tryptophan and phenolic compounds, derived from both endogenous and colonic microbial metabolism. Furthermore, potential inter-species metabolic differences were studied. For this purpose, 29 healthy subjects were allocated to a high (n = 14) or low protein diet (n = 15) for 2 weeks. In addition, 20 wild-type FVB mice were randomized to a high protein or control diet for 21 days. Plasma and urine samples were analyzed with liquid chromatography-mass spectrometry for measurement of tryptophan and phenolic metabolites. In human subjects, we observed significant changes in plasma level and urinary excretion of indoxyl sulfate (P 0.004 and P 0.001), and in urinary excretion of indoxyl glucuronide (P 0.01), kynurenic acid (P 0.006) and quinolinic acid (P 0.02). In mice, significant differences were noted in plasma tryptophan (P 0.03), indole-3-acetic acid (P 0.02), p-cresyl glucuronide (P 0.03), phenyl sulfate (P 0.004) and phenylacetic acid (P 0.01). Thus, dietary protein intake affects plasma levels and generation of various mammalian metabolites, suggesting an influence on both endogenous and colonic microbial metabolism. Metabolite changes are dissimilar between human subjects and mice, pointing to inter-species metabolic differences with respect to protein intake.
URI: 
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Nephrology
Translational Research in GastroIntestinal Disorders
Drug Delivery and Disposition
× corresponding author
# (joint) last author

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