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Title: Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers
Authors: Hendrix, Jelle
Baumgärtel, Viola
Schrimpf, Waldemar
Ivanchenko, Sergey
Digman, Michelle A
Gratton, Enrico
Kräusslich, Hans-Georg
Müller, Barbara
Lamb, Don C # ×
Issue Date: Aug-2015
Publisher: Rockefeller Institute Press
Series Title: Journal of Cell Biology vol:210 issue:4 pages:629-646
Article number: 10.1083/jcb.201504006
Abstract: Assembly of the Gag polyprotein into new viral particles in infected cells is a crucial step in the retroviral replication cycle. Currently, little is known about the onset of assembly in the cytosol. In this paper, we analyzed the cytosolic HIV-1 Gag fraction in real time in live cells using advanced fluctuation imaging methods and thereby provide detailed insights into the complex relationship between cytosolic Gag mobility, stoichiometry, and interactions. We show that Gag diffuses as a monomer on the subsecond timescale with severely reduced mobility. Reduction of mobility is associated with basic residues in its nucleocapsid (NC) domain, whereas capsid (CA) and matrix (MA) domains do not contribute significantly. Strikingly, another diffusive Gag species was observed on the seconds timescale that oligomerized in a concentration-dependent manner. Both NC- and CA-mediated interactions strongly assist this process. Our results reveal potential nucleation steps of cytosolic Gag fractions before membrane-assisted Gag assembly.
URI: 
ISSN: 0021-9525
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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