Title: Efficient recombinase mediated cassette exchange in hPSC to study the hepatic lineage reveals AAVS1 locus-mediated transgene inhibition
Authors: Ordovas Vidal, Laura
Pistoni, Elena
Boon, Ruben
Chen, Yemiao
Sambathkumar, Rangarajan
Bobis-Wozowicz, S
Helsen, Nicky
Vanhove, Jolien
Berckmans, Pieter
Cai, Qing
Vanuytsel, Kim
Wolfs, Esther
Eggermont, Kristel
Vanslembrouck, Veerle
Schmidt, Bela Z
Raitano, Susanna
Struys, T
Guo, Wenting
Van Den Bosch, Ludo
Nahmias, Y
Cathomen, T
Verfaillie, Catherine
Issue Date: 20-Apr-2015
Publisher: Program Interuniversity Stem Cell Meeting
Conference: Program Interuniversity Stem Cell Meeting date:20 April 2015
Article number: 13
Abstract: Tools for rapid and efficient transgenesis in “safe harbour”•loci in an isogenic context remain important to fully exploit the possibilities of human pluripotent stem cells (hPSC). We created hPSC master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 safe harbour locus. RMCE using positive/negative selection allows generation of transgenic lines in 15 days with 100% targeting efficiency and without random integrations. Using RMCE, we successfully incorporated a number of transgenes useful for lineage identification, assessment of cell toxicity, and gene over-expression. However, we observed unexpected and variable inhibition of expression of some of the transgenes in the AAVS1 locus, caused by DNA methylation and other unknown mechanisms, in undifferentiated hESC and during hepatic differentiation. Therefore, the AAVS1 locus cannot be considered a universally safe harbour locus, at least for the hepatic lineage, and using it for transgenesis in human cells will require thorough assessment of promoter function.
Publication status: published
KU Leuven publication type: AMa
Appears in Collections:Interdepartemental Stem Cell Institute (-)
Embryo and Stemcells (-)
Stem Cell Biology and Embryology (+)
Biomedical MRI
Laboratory of Neuronal Communication
Laboratory for Neurobiology

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