Title: Characterization of Human Duodenal Fluids in Fasted and Fed State Conditions
Authors: Riethorst, Danny ×
Mols, Raf
Duchateau, Guus
Tack, Jan
Brouwers, Joachim
Augustijns, Patrick #
Issue Date: Feb-2016
Publisher: American Chemical Society and American Pharmaceutical Association
Series Title: Journal of Pharmaceutical Sciences vol:105 issue:2 pages:673-681
Article number: 10.1002/jps.24603
Abstract: This work provides an elaborate characterization of human intestinal fluids (HIF) collected in fasted- and fed-state conditions. HIF from 20 healthy volunteers (10 M/F) were aspirated by intubation near the ligament of Treitz in a time-dependent manner (10-min intervals) and characterized for pH, bile salts, phospholipids, cholesterol, triacylglycerides (TAG), diacylglycerides (DAG), monoacylglycerides (MAG), free fatty acids (FFA), pancreatic lipase, phospholipase A2, and nonspecific esterase activity. For almost all parameters, a food-induced effect was observed. Results were characterized by a high variability, as illustrated by the broad ranges observed for each parameter: pH (fasted: 3.4-8.3; fed: 4.7-7.1), bile salts (fasted: 0.03-36.18 mM; fed: 0.74-86.14 mM), phospholipids (fasted: 0.01-6.33 mM; fed: 0.16-14.39 mM), cholesterol (fasted: 0-0.48 mM; fed: 0-3.29 mM), TAG (fed: 0-6.76 mg/mL), DAG (fed: 0-3.64 mg/mL), MAG (fasted: 0-1.09 mg/mL; fed: 0-11.36 mg/mL), FFA (fasted: 0-3.86 mg/mL; fed: 0.53-15.0 mg/mL), pancreatic lipase (fasted: 26-86 μg/mL; fed: 146-415 μg/mL), phospholipase A2 (fasted: 3-6 ng/mL; fed: 4.3-27.7 ng/mL), and nonspecific esterase activity (fasted: 270-4900 U/mL; fed: 430-4655 U/mL). This comprehensive overview may serve as reference data for physiologically based pharmacokinetic modeling and the optimization of biorelevant simulated intestinal fluids for the use in in vitro dissolution, solubility, and permeability profiling. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.
ISSN: 0022-3549
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Drug Delivery and Disposition
Translational Research in GastroIntestinal Disorders
× corresponding author
# (joint) last author

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