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Title: Development of a molecular subtyping method for Salmonella Typhimurium combining different types of markers in a Luminex xTAG assay
Authors: Wuyts, VĂ©ronique
Bertrand, Sophie
Roosens, Nancy
Marchal, Kathleen
De Keersmaecker, Sigrid
Issue Date: Jun-2013
Conference: xSAMPLES Benelux location:Rotterdam, The Netherlands date:19-20 June 2013
Abstract: Subtyping of Salmonella enterica serovar Typhimurium, a frequent cause of food-borne diseases, is critical for surveillance and identification, tracking and ultimately confinement of outbreaks. We present a proof of concept of a molecular subtyping method for S. Typhimurium which combines different types of markers in a multiplex assay with detection by the Luminex xTAG technology in a high-throughput format. Selected markers include, amongst others, markers based on AFLP fragments, prophage genomes, sequence repeats, antibiotic resistance genes and SNPs.
The optimal multiplex design was evaluated and a ligation dependent amplification (LDA) assay was found to be the most efficient strategy to target the selected markers. The in vitro stability of the selected markers was verified. To determine the discriminatory ability, S. Typhimurium isolates of most common phage types in Belgium were subjected to the proposed subtyping method. The capability to identify outbreaks was tested with strains from two outbreaks in Belgium that occurred in 2008 and 2011. The resulting profiles were examined for correlation with phage types, MLVA and antimicrobial resistance profiles. The potential of the method based on the xTAG technology to provide a new subtyping scheme for S. Typhimurium will be discussed.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Centre of Microbial and Plant Genetics

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