Title: Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming
Authors: Jullien, Jerome *
Miyamoto, Kei *
Pasque, Vincent *
Allen, George E
Bradshaw, Charles R
Garrett, Nigel J
Halley-Stott, Richard P
Kimura, Hiroshi
Ohsumi, Keita
Gurdon, John B # ×
Issue Date: Aug-2014
Publisher: Cell Press
Series Title: Molecular Cell vol:55 issue:4 pages:524-36
Article number: 10.1016/j.molcel.2014.06.024
Abstract: Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a selective switch in transcription toward an oocyte rather than pluripotent type, without requiring new protein synthesis. Time-course analyses at the single-nucleus level show that transcriptional reprogramming is induced in most transplanted nuclei in a highly hierarchical manner. We demonstrate that an extensive exchange of somatic- for oocyte-specific factors mediates reprogramming and leads to robust oocyte RNA polymerase II binding and phosphorylation on transplanted chromatin. Moreover, genome-wide binding of oocyte-specific linker histone B4 supports its role in transcriptional reprogramming. Thus, our study reveals the rapid, abundant, and stepwise loading of oocyte-specific factors onto somatic chromatin as important determinants for successful reprogramming.
ISSN: 1097-2765
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
* (joint) first author
× corresponding author
# (joint) last author

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