Title: Analysis of the stabilizing effect of epsilon-aminocaproic acid by electrophoretic techniques and immunoblotting
Authors: Van Hoeyveld, Ernestina ×
Lejoly, M
Walravens, M J
Stevens, E A #
Issue Date: Mar-1989
Publisher: Elsevier
Series Title: Journal of Allergy and Clinical Immunology vol:83 issue:3 pages:601-9
Abstract: The effect of epsilon-aminocaproic acid (EACA) on the degradation of aqueous pollen extracts was studied by isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The extracts were stored at 4 degrees C for 7 days and at 37 degrees C for 1, 4, and 7 days. Addition of 0.1 mol/L of EACA before storage partly protected the extract from degradation. The protective effect of EACA could be demonstrated most clearly by immunoblotting, suggesting that more epitopes were preserved in an antigenic configuration. The stabilizing effect increased with higher EACA concentrations.
ISSN: 0091-6749
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Microbiology & Immunology - miscellaneous
Laboratory of Clinical Immunology
× corresponding author
# (joint) last author

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