Author:

Keywords:

beta vulgaris l., cold acclimation, cryopreservation, fatty acids, sugars, encapsulation-dehydration technique, low-temperature, lipid-composition, plasma-membranes, abscisic-acid, tips, n,n-dimethylformamide, desiccation, tolerance, cultures, Science & Technology, Life Sciences & Biomedicine, Plant Sciences, Beta vulgaris L., ENCAPSULATION-DEHYDRATION TECHNIQUE, LOW-TEMPERATURE, LIPID-COMPOSITION, PLASMA-MEMBRANES, ABSCISIC-ACID, TIPS, N,N-DIMETHYLFORMAMIDE, DESICCATION, TOLERANCE, CULTURES, 0607 Plant Biology, 0703 Crop and Pasture Production, Plant Biology & Botany, 3001 Agricultural biotechnology, 3108 Plant biology

Abstract:

To cryopreserve sugar beet shoot tips using an encapsulation-dehydration technique, cold hardening of in vitro plants was needed to obtain high survival rates after freezing. Cold acclimation not only enhanced dehydration and freezing tolerance, but also induced several changes in sugar beet shoots. Plants contained greater amounts of sucrose, D-glucose and D-fructose and the fatty acid composition of lipids changed. Furthermore, the unsaturation level of membrane lipids, estimated by the (C-18:2 +C-18:1)/C-16:0 ratio, increased after cold hardening. These changes were correlated with better survival rates after cryopreservation.