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Title: Improved detection of β-N-methylamino-L-alanine using N-hydroxysuccinimide ester of N-butylnicotinic acid for the localization of BMAA in blue mussels (Mytilus edulis)
Authors: Andrýs, Rudolf *
Zurita, Javier *
Zguna, Nadezda
Verschueren, Klaas
De Borggraeve, Wim
Ilag, Leopold L # ×
Issue Date: May-2015
Publisher: Springer-Verlag
Series Title: Analytical and Bioanalytical Chemistry vol:407 issue:13 pages:3743-50
Abstract: β-N-Methylamino-L-alanine (BMAA) is an important non-protein amino acid linked to neurodegenerative diseases, specifically amyotrophic lateral sclerosis (ALS). Because it can be transferred and bioaccumulated higher up the food chain, it poses significant public health concerns; thus, improved detection methods are of prime importance for the identification and management of these toxins. Here, we report the successful use of N-hydroxysuccinimide ester of N-butylnicotinic acid (C4-NA-NHS) for the efficient separation of BMAA from its isomers and higher sensitivity in detecting BMAA compared to the current method of choice using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization. Implementation of this efficient method allowed localization of BMAA in the non-visceral tissues of blue mussels, suggesting that more efficient depuration may be required to remove this toxin prior to consumption. This is a crucial method in establishing the absence or presence of the neurotoxic amino acid BMAA in food, environmental or biomedical samples.
ISSN: 1618-2642
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Design and Synthesis
* (joint) first author
× corresponding author
# (joint) last author

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