Title: Identification of novel radiation-induced p53-dependent transcripts extensively regulated during mouse brain development
Authors: Quintens, Roel ×
Verreet, Tine
Janssen, Ann
Neefs, Mieke
Leysen, Liselotte
Michaux, Arlette
Verslegers, Mieke
Samari, Nada
Pani, Giuseppe
Verheyde, Joris
Baatout, Sarah
Benotmane, Mohammed A #
Issue Date: 2015
Publisher: The Company of Biologists Ltd.
Series Title: Biology Open vol:4 issue:3 pages:331-44
Article number: 10.1242/bio.20149969
Abstract: Ionizing radiation is a potent activator of the tumor suppressor gene p53, which itself regulates the transcription of genes involved in canonical pathways such as the cell cycle, DNA repair and apoptosis as well as other biological processes like metabolism, autophagy, differentiation and development. In this study, we performed a meta-analysis on gene expression data from different in vivo and in vitro experiments to identify a signature of early radiation-responsive genes which were predicted to be predominantly regulated by p53. Moreover, we found that several genes expressed different transcript isoforms after irradiation in a p53-dependent manner. Among this gene signature, we identified novel p53 targets, some of which have not yet been functionally characterized. Surprisingly, in contrast to genes from the canonical p53-regulated pathways, our gene signature was found to be highly enriched during embryonic and post-natal brain development and during in vitro neuronal differentiation. Furthermore, we could show that for a number of genes, radiation-responsive transcript variants were upregulated during development and differentiation, while radiation non-responsive variants were not. This suggests that radiation exposure of the developing brain and immature cortical neurons results in the p53-mediated activation of a neuronal differentiation program. Overall, our results further increase the knowledge of the radiation-induced p53 network of the embryonic brain and provide more evidence concerning the importance of p53 and its transcriptional targets during mouse brain development.
ISSN: 2046-6390
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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