Title: Antigen excess in modern immunoassays: to anticipate on the unexpected
Authors: Jacobs, Joannes F M ×
van der Molen, Renate G
Bossuyt, Xavier
Damoiseaux, Jan #
Issue Date: Feb-2015
Publisher: Elsevier
Series Title: Autoimmunity Reviews vol:14 issue:2 pages:160-7
Article number: 10.1016/j.autrev.2014.10.018
Abstract: Immunoassays measuring sera with high analyte concentration may be prone to an artifact that causes underestimation of the analyte concentration. This phenomenon is generally described as antigen excess or the prozone effect. Characteristically, serum with high concentrations of a certain analyte can give a false negative/low result when tested at the recommended dilution, but reacts strongly positive upon further dilution. Increased insight of the antigen excess mechanisms and tools to prevent it has reduced the analytical problems caused by prozone effects in daily laboratory practice. However, misinterpretation of laboratory results caused by antigen excess does still occur, in virtually any type of immunoassay. Awareness by the laboratory specialist of the mechanisms underlying antigen excess in the different immunoassays, strategies to detect it, and adequate communication with clinicians can help to avoid reporting false negative test-results.
ISSN: 1568-9972
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Experimental Laboratory Immunology
× corresponding author
# (joint) last author

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