Title: Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture
Authors: Noulin, Florian ×
Manesia, Javed Karim
Rosanas-Urgell, Anna
Erhart, Annette
Borlon, Céline
Van Den Abbeele, Jan
d'Alessandro, Umberto
Verfaillie, Catherine #
Issue Date: 2014
Publisher: Public Library of Sciene
Series Title: PLoS One vol:9 issue:11 pages:e112496
Article number: 10.1371/journal.pone.0112496
Abstract: The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Stem Cell Biology and Embryology (+)
× corresponding author
# (joint) last author

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