Title: Cell membrane damage is involved in the impaired survival of bone marrow stem cells by oxidized low-density lipoprotein
Authors: Li, Xin ×
Xiao, Yuan
Cui, Yuqi
Tan, Tao
Narasimhulu, Chandrakala A
Hao, Hong
Liu, Lingjuan
Zhang, Jia
He, Guanglong
Verfaillie, Catherine
Lei, Minxiang
Parthasarathy, Sampath
Ma, Jianjie
Zhu, Hua
Liu, Zhenguo #
Issue Date: Dec-2014
Publisher: Wiley-Blackwell Publishing Ltd.
Series Title: Journal of Cellular and Molecular Medicine vol:18 issue:12 pages:2445-53
Article number: 10.1111/jcmm.12424
Abstract: Cell therapy with bone marrow stem cells (BMSCs) remains a viable option for tissue repair and regeneration. A major challenge for cell therapy is the limited cell survival after implantation. This study was to investigate the effect of oxidized low-density lipoprotein (ox-LDL, naturally present in human blood) on BMSC injury and the effect of MG53, a tissue repair protein, for the improvement of stem cell survival. Rat bone marrow multipotent adult progenitor cells (MAPCs) were treated with ox-LDL, which caused significant cell death as reflected by the increased LDH release to the media. Exposure of MAPCs to ox-LDL led to entry of fluorescent dye FM1-43 measured under confocal microscope, suggesting damage to the plasma membrane. Ox-LDL also generated reactive oxygen species (ROS) as measured with electron paramagnetic resonance spectroscopy. While antioxidant N-acetylcysteine completely blocked ROS production from ox-LDL, it failed to prevent ox-LDL-induced cell death. When MAPCs were treated with the recombinant human MG53 protein (rhMG53) ox-LDL induced LDH release and FM1-43 dye entry were significantly reduced. In the presence of rhMG53, the MAPCs showed enhanced cell survival and proliferation. Our data suggest that membrane damage induced by ox-LDL contributed to the impaired survival of MAPCs. rhMG53 treatment protected MAPCs against membrane damage and enhanced their survival which might represent a novel means for improving efficacy for stem cell-based therapy for treatment of diseases, especially in setting of hyperlipidemia.
ISSN: 1582-4934
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Stem Cell Biology and Embryology (+)
× corresponding author
# (joint) last author

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