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Title: The Wnt/β-catenin signaling pathway tips the balance between apoptosis and reprograming of cell fusion hybrids
Authors: Lluis, Frederic
Pedone, Elisa
Pepe, Stefano
Cosma, Maria Pia # ×
Issue Date: Nov-2010
Publisher: AlphaMed Press
Series Title: Stem Cells vol:28 issue:11 pages:1940-9
Article number: 10.1002/stem.515
Abstract: Cell-cell fusion contributes to cell differentiation and developmental processes. We have previously showed that activation of Wnt/β-catenin enhances somatic cell reprograming after polyethylene glycol (PEG)-mediated fusion. Here, we show that neural stem cells and ESCs can fuse spontaneously in cocultures, although with very low efficiency (about 2%), as the hybrids undergo apoptosis. In contrast, when Wnt/β-catenin signaling is activated in ESCs and leads to accumulation of low amounts of β-catenin in the nucleus, activated ESCs can reprogram somatic cells with very high efficiency after spontaneous fusion. Furthermore, we also show that different levels of β-catenin accumulation in the ESC nuclei can modulate cell proliferation, although in our experimental setting, cell proliferation does not modulate the reprograming efficiency per se. Overall, the present study provides evidence that spontaneous fusion occurs, while the survival of the reprogramed clones is strictly dependent on induction of a Wnt-mediated reprograming pathway.
ISSN: 1066-5099
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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