A roadmap for interpreting (13)C metabolite labeling patterns from cells

Buescher, Joerg M; Antoniewicz, Maciek R; Boros, Laszlo G; Burgess, Shawn C; Brunengraber, Henri; Clish, Clary B; DeBerardinis, Ralph J; Feron, Olivier; Frezza, Christian; Ghesquiere, Bart; Gottlieb, Eyal; Hiller, Karsten; Jones, Russell G; Kamphorst, Jurre J; Kibbey, Richard G; Kimmelman, Alec C; Locasale, Jason W; Lunt, Sophia Y; Maddocks, Oliver Dk; Malloy, Craig; Metallo, Christian M; Meuillet, Emmanuelle J; Munger, Joshua; Nöh, Katharina; Rabinowitz, Joshua D; Ralser, Markus; Sauer, Uwe; Stephanopoulos, Gregory; St-Pierre, Julie; Tennant, Daniel A; Wittmann, Christoph; Vander Heiden, Matthew G; Vazquez, Alexei; Vousden, Karen; Young, Jamey D; Zamboni, Nicola; Fendt, Sarah-Maria

doi:10.1016/j.copbio.2015.02.003

A roadmap for interpreting (13)C metabolite labeling patterns from cells

Author:

Buescher, Joerg M

Antoniewicz, Maciek R ; Boros, Laszlo G ; Burgess, Shawn C ; Brunengraber, Henri ; Clish, Clary B ; DeBerardinis, Ralph J ; Feron, Olivier ; Frezza, Christian ; Ghesquiere, Bart ; Gottlieb, Eyal ; Hiller, Karsten ; Jones, Russell G ; Kamphorst, Jurre J ; Kibbey, Richard G ; Kimmelman, Alec C ; Locasale, Jason W ; Lunt, Sophia Y ; Maddocks, Oliver Dk ; Malloy, Craig ; Metallo, Christian M ; Meuillet, Emmanuelle J ; Munger, Joshua ; Nöh, Katharina ; Rabinowitz, Joshua D ; Ralser, Markus ; Sauer, Uwe ; Stephanopoulos, Gregory ; St-Pierre, Julie ; Tennant, Daniel A ; Wittmann, Christoph ; Vander Heiden, Matthew G ; Vazquez, Alexei ; Vousden, Karen ; Young, Jamey D ; Zamboni, Nicola ; Fendt, Sarah-Maria

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, REDUCTIVE GLUTAMINE-METABOLISM, MASS ISOTOPOMER DISTRIBUTIONS, PENTOSE-PHOSPHATE PATHWAY, FATTY-ACID SYNTHESIS, FLUX ANALYSIS, CORYNEBACTERIUM-GLUTAMICUM, ALPHA-KETOGLUTARATE, TCA CYCLE, RAT-LIVER, GROWTH, Animals, Carbon Isotopes, Cell Survival, Humans, Isotope Labeling, Metabolic Networks and Pathways, 06 Biological Sciences, 09 Engineering, 10 Technology, Biotechnology, 3001 Agricultural biotechnology, 3106 Industrial biotechnology, 3206 Medical biotechnology

Abstract:

Measuring intracellular metabolism has increasingly led to important insights in biomedical research. (13)C tracer analysis, although less information-rich than quantitative (13)C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting (13)C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.