Author:

Keywords:

His-tagged protein immobilization, Surface plasmon resonance biosensors, Dipyrromethene-Cu(II), Pentetic acid thiol ligand-Cu(II), Janus Kinase 2, Kinetic and thermodynamic analysis, Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, SELF-ASSEMBLED MONOLAYERS, ELECTRODE-THE BASE, ORIENTED IMMOBILIZATION, PROTEIN, BINDING, DOMAINS, BIOSENSORS, THROMBIN, AFFINITY, APTAMERS, Biosensing Techniques, Coordination Complexes, Copper, Dextrans, Gold, Histidine, Humans, Kinetics, Sulfhydryl Compounds, Surface Plasmon Resonance, Surface Properties, Thermodynamics, 0301 Analytical Chemistry, 0399 Other Chemical Sciences, Analytical Chemistry, 3401 Analytical chemistry

Abstract:

New surface plasmon resonance (SPR) sensing platforms which consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His6-JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His6-JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.