Title: A substrate-inspired probe monitors translocation, activation, and subcellular targeting of bacterial type III effector protease AvrPphB
Authors: Lu, Haibin ×
Wang, Zheming
Shabab, Mohammed
Oeljeklaus, Julian
Verhelst, Steven
Kaschani, Farnusch
Kaiser, Markus
Bogyo, Matthew
van der Hoorn, Renier A L #
Issue Date: Feb-2013
Publisher: Current Biology Ltd.
Series Title: Chemistry & Biology vol:20 issue:2 pages:168-76
Article number: 10.1016/j.chembiol.2012.11.007
Abstract: The AvrPphB effector of Pseudomonas syringae is a papain-like protease that is injected into the host plant cell and cleaves specific kinases to disrupt immune signaling. Here, we used the unique substrate specificity of AvrPphB to generate a specific activity-based probe. This probe displays various AvrPphB isoforms in bacterial extracts, upon secretion and inside the host plant. We show that AvrPphB is secreted as a proprotease and that secretion requires the prodomain, but probably does not involve a pH-dependent unfolding mechanism. The prodomain removal is required for the ability of AvrPphB to trigger a hypersensitive cell death in resistant host plants, presumably since processing exposes a hidden acylation site required for subcellular targeting in the host cell. We detected two active isoforms of AvrPphB in planta, of which the major one localizes exclusively to membranes.
ISSN: 1074-5521
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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