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Title: Performance of different culture methods and of a commercial molecular assay for the detection of carbapenemase-producing Enterobacteriaceae in nursing homes and rehabilitation centers
Authors: Saegeman, Veroniek ×
Van den Eynde, J
Niclaes, L
De Ridder, Dirk
Schuermans, Annette
Glupczynski, Y #
Issue Date: Jan-2015
Publisher: Springer-Verlag
Series Title: European Journal of Clinical Microbiology & Infectious Diseases vol:1 issue:1 pages:1-7
Abstract: Over the last years carbapenemase-producing Enterobacteriaceae (CPE) have been
increasingly detected among patients in acute care hospitals but also in long-term
care facilities.
In this point prevalence survey, residents from three nursing homes and patients in one
rehabilitation center were screened for asymptomatic intestinal carriage of CPE by
rectal swabs. The first objective was to evaluate the hypothesis of the establishment of
a CPE reservoir in a geriatric/chronic care population. Secondly, we evaluated the
comparative performances of different culture methods (ChromID®CARBA,
ChromID®OXA-48, MacConkey with temocillin/meropenem, ertapenem enrichment
broth) and of a commercial molecular assay (Check-Direct CPE).
From the 257 included residents, only one had evidence for CPE carriage. From the
rectal swabs of this resident, an OXA-48 producing Klebsiella pneumoniae could be
isolated and was confirmed by a molecular assay both on the strain and on the rectal
swab. Specificity of the different culture methods and Check-Direct CPE PCR was at
least 97%. Neither enrichment broth nor prolonged incubation up to 48 hours did
increase the yield of CPE.
This point prevalence survey shows a low CPE prevalence of 0.39%. Larger scaled
studies are needed to confirm the role of chronic care settings as secondary CPE
ISSN: 0934-9723
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Organ Systems (+)
Laboratory of Clinical Bacteriology and Mycology
Environment and Health - miscellaneous
× corresponding author
# (joint) last author

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