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Title: Intermediate filament structure: the bottom-up approach
Authors: Chernyatina, Anastasia
Guzenko, Dmytro
Strelkov, Sergei # ×
Issue Date: Feb-2015
Publisher: Current Science
Series Title: Current Opinion in Cell Biology vol:32C pages:65-72
Article number: S0955-0674(14)00148-3
Abstract: Intermediate filaments (IFs) result from a key cytoskeletal protein class in metazoan cells, but currently there is no consensus as to their three-dimensional architecture. IF proteins form elongated dimers based on the coiled-coil structure within their central 'rod' domain. Here we focus on the atomic structure of this elementary dimer, elucidated using X-ray crystallography on multiple fragments and electron paramagnetic resonance experiments on spin-labelled vimentin samples. In line with conserved sequence features, the rod of all IF proteins is composed of three coiled-coil segments containing heptad and hendecad repeats and interconnected by linkers. In addition, the next assembly intermediate beyond the dimer, the tetramer, could be modelled. The impact of these structural results towards understanding the assembly mechanism is discussed.
URI: 
ISSN: 0955-0674
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
× corresponding author
# (joint) last author

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