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Title: Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging
Authors: Lescale, C ×
Schenten, V
Djeghoul, D
Bennabi, M
Gaignier, F
Vandamme, Katleen
Strazielle, C
Kuzniak, I
Petite, H
Dosquet, C
Frippiat, JP
Goodhardt, M #
Issue Date: 5-Nov-2014
Publisher: The Federation of American Societies for Experimental Biology
Series Title: FASEB Journal vol:29 issue:2 pages:455-463
Article number: pii:fj.14-259770
Abstract: Within the bone marrow, the endosteal niche plays a crucial role in B-cell differentiation. Because spaceflight is associated with osteoporosis, we investigated whether changes in bone microstructure induced by a ground-based model of spaceflight, hind limb unloading (HU), could affect B lymphopoiesis. To this end, we analyzed both bone parameters and the frequency of early hematopoietic precursors and cells of the B lineage after 3, 6, 13, and 21 d of HU. We found that limb disuse leads to a decrease in both bone microstructure and the frequency of B-cell progenitors in the bone marrow. Although multipotent hematopoietic progenitors were not affected by HU, a decrease in B lymphopoiesis was observed as of the common lymphoid progenitor (CLP) stage with a major block at the progenitor B (pro-B) to precursor B (pre-B) cell transition (5- to 10-fold decrease). The modifications in B lymphopoiesis were similar to those observed in aged mice and, as with aging, decreased B-cell generation in HU mice was associated with reduced expression of B-cell transcription factors, early B-cell factor (EBF) and Pax5, and an alteration in STAT5-mediated IL-7 signaling. These findings demonstrate that mechanical unloading of hind limbs results in a decrease in early B-cell differentiation resembling age-related modifications in B lymphopoiesis.
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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