Title: Primary Hepatocytes in Sandwich Culture
Other Titles: Sandwich-Cultured Hepatocytes
Authors: Keemink, Janneke
Oorts, Marlies
Annaert, Pieter
Series Editors: Walker, John
Issue Date: 2015
Publisher: Humana Press
Series Title: Methods in Molecular Biology vol:1250
Host Document: Protocols in In Vitro Hepatocyte Research edition:1st pages:175-188
Article number: 12
Abstract: Hepatocytes in sandwich configuration constitute of primary hepatocytes cultured between two layers of extracellular matrix. Sandwich-cultured hepatocytes maintain expression of liver-specific proteins and gradually form intact bile canaliculi with functional biliary excretion of endogenous compounds and xenobiotics. Both freshly isolated and cryopreserved hepatocytes can be used to establish sandwich cultures. Therefore, this preclinical model has become an invaluable in vitro tool to evaluate hepatobiliary drug transport, metabolism, hepatotoxicity, and drug interactions. In this chapter, commonly used procedures to cultivate primary hepatocytes from human and rat in sandwich configuration are described.
ISBN: 978-1-4939-2073-0
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IHb
Appears in Collections:Drug Delivery and Disposition

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