Title: Potato phosphorylase catalyzed synthesis of amylose-lipid complexes
Authors: Gelders, GG ×
Goesaert, Hans
Delcour, Jan #
Issue Date: 2005
Publisher: Amer chemical soc
Series Title: Biomacromolecules vol:6 issue:5 pages:2622-2629
Abstract: On-line size-exclusion chromatography monitoring of potato phosphorylase catalyzed amylose synthesis-starting from alpha-D-glucose-1-P and maltohexaose-revealed rather monodisperse amylose populations. In the presence of lipids, amylose-lipid complexes spontaneously formed and precipitated. They were recovered by centrifugation, freeze-dried, and characterized by wide-angle X-ray diffraction and differential scanning calorimetry. The presence of lipids during amylose synthesis led to lower amylose degrees of polymerization (DP). Lipid chain length defined amylose DP, which increased in the order myristic acid (C14), glyceryl monostearate (GMS), stearic acid (C18), and docosanoic acid (C22). The thermal stability of the complexes increased in the same manner, with the C22 complexes having the highest dissociation temperature. In addition, we hypothesized that these results provide additional evidence for the fringed micellar organization of (semienzymically synthesized) amylose-lipid complexes.
ISSN: 1525-7797
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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