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Title: Cloning, constitutive activity and expression profiling of two receptors related to relaxin receptors in Drosophila melanogaster
Authors: Van Hiel, Matthias B ×
Vandersmissen, Hans Peter
Proost, Paul
Vanden Broeck, Jozef #
Issue Date: Jun-2015
Publisher: Elsevier
Series Title: Peptides vol:68 pages:83-90
Abstract: Leucine-rich repeat containing G protein-coupled receptors (LGRs) comprise a cluster of transmembrane proteins, characterized by the presence of a large N-terminal extracellular domain. This receptor group can be classified into three subtypes. Belonging to the subtype C LGRs are the mammalian relaxin receptors LGR7 (RXFP1) and LGR8 (RXFP2), which mediate important reproductive and other processes. We identified two related receptors in the genome of the fruit fly and cloned their open reading frames into an expression vector. Interestingly, dLGR3 demonstrated constitutive activity at very low doses of transfected plasmid, whereas dLGR4 did not show any basal activity. Both receptors exhibited a similar expression pattern during development, with relatively high transcript levels during the first larval stage. In addition, both receptors displayed higher expression in male adult flies as compared to female flies. Analysis of the tissue distribution of both receptor transcripts revealed a high expression of dLGR3 in the female fat body, while the expression of dLGR4 peaked in the midgut of both the wandering and adult stage.
URI: 
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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