Title: In vitro construction of lacZ gene fusions with the nifHDK-operon of Azospirillum brasilense
Authors: Milcamps, A ×
Vanderleyden, Jozef #
Issue Date: 1991
Publisher: Elsevier science bv
Series Title: Fems Microbiology Letters vol:77 issue:1 pages:79-84
Abstract: Site-directed mutagenesis with Tn3-HoHo1 was performed on pAB35, a pRK290 vector with the nifHDK operon of Azospirillum brasilense strain Sp7. No transcriptional readthrough from vector sequences into the insert DNA was detected upon tranfer into Azospirillum. A nifH::lacZ and a nifD::lacZ translational fusion were obtained and characterized by Western blotting with a monoclonal antibody against beta-galactosidase. Detectable beta-galactosidase activities of A. brasilense transconjugants were only observed under nitrogen derepressed conditions.
ISSN: 0378-1097
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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