An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum
Bock, Thomas × Chen, Wei-Hua Ori, Alessandro Malik, Nayab Silva-Martin, Noella Huerta-Cepas, Jaime Powell, Sean T Kastritis, Panagiotis L Smyshlyaev, Georgy Vonkova, Ivana Kirkpatrick, Joanna Doerks, Tobias Nesme, Leo Baßler, Jochen Kos, Martin Hurt, Ed Carlomagno, Teresa Gavin, Anne-Claude Barabas, Orsolya Müller, Christoph W van Noort, Vera Beck, Martin Bork, Peer #
Oxford University Press
Nucleic Acids Research vol:42 issue:22 pages:13525-13533
The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.