Title: Toxicity of nanoparticles embedded in paints compared to pristine nanoparticles, in vitro study
Authors: Smulders, Stijn ×
Luyts, Katrien
Brabants, Gert
Golanski, Luana
Martens, Johan
Vanoirbeek, Jeroen
Hoet, Peter #
Issue Date: Jan-2015
Publisher: Elsevier/North-Holland
Series Title: Toxicology Letters vol:232 issue:2 pages:333-339
Abstract: The unique physicochemical properties of nanomaterials has led to an increased use in the paint and coating industry. In this study, the in vitro toxicity of three pristine ENPs (TiO2, Ag and SiO2), three aged paints containing ENPs (TiO2, Ag and SiO2) and control paints without ENPs were compared. In a first experiment, cytotoxicity was assessed using a biculture consisting of human bronchial epithelial (16HBE14o-) cells and human monocytic cells (THP-1) to determine subtoxic concentrations. In a second experiment, a new coculture model of the lung-blood barrier consisting of 16HBE14o- cells, THP-1 and human lung microvascular endothelial cells (HLMVEC) was used to study pulmonary and extrapulmonary toxicity. The results show that the pristine TiO2 and Ag ENPs have some cytotoxic effects at relative high dose, while pristine SiO2 ENPs and all aged paints with ENPs and control paints do not. In the complex triculture model of the lung-blood barrier, no considerable changes were observed after exposure to subtoxic concentration of the different pristine ENPs and paint particles. In conclusion, we demonstrated that although pristine ENPs show some toxic effects, no significant toxicological effects were observed when they were embedded in a complex paint matrix.
ISSN: 0378-4274
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Surface Chemistry and Catalysis
Environment and Health - miscellaneous
Experimental Laboratory Immunology
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
2015 Smulders S et al Tox Lett.pdf Published 791KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science