Title: Tau Monoclonal Antibody Generation based on Humanized Yeast Models: Impact on Tau Oligomerization and Diagnostics
Authors: Rosseels, Joëlle *
Van den Brande, Jeff *
Violet, Marie *
Jacobs, Dirk
Grognet, Pierre
Lopez, Juan
Huvent, Isabelle
Caldara, Marina
Swinnen, Erwin
Papegaey, Anthony
Caillierez, Raphaëlle
Buée-Scherrer, Valerie
Engelborghs, Sebastiaan
Lippens, Guy
Colin, Morvane
Buée, Luc
Galas, Marie-Christine
Vanmechelen, Eugeen #
Winderickx, Joris # ×
Issue Date: Feb-2015
Publisher: American Society for Biochemistry and Molecular Biology
Series Title: Journal of Biological Chemistry vol:290 issue:7 pages:4059-4074
Article number: jbc.M114.627919
Abstract: A link between Tau phosphorylation and aggregation has been shown in different models for Alzheimer disease, including yeast. We used human Tau purified from yeast models to generate new monoclonal antibodies, of which three were further characterized. The first antibody, ADx201, binds the Tau proline-rich region independently of the phosphorylation status, while the second, ADx215, detects an epitope formed by the Tau N-terminus when Tau is not phosphorylated at Tyr18. For the third antibody, ADx210, the binding site could not be determined since its epitope is probably conformational. All three antibodies stained tangle-like structures in different brain sections of THY-Tau22 transgenic mice and Alzheimer patients and ADx201 and ADx210 also detected neuritic plaques in the cortex of the patient brains. In hippocampal homogenates from THY-Tau22 mice and cortex homogenates obtained from Alzheimer patients, ADx215 consistently stained specific low-order Tau oligomers in diseased brain, which in size correspond to Tau dimers. ADx201 and ADx210 additionally reacted to higher-order Tau oligomers and presumed prefibrillar structures in the patient samples. Our data further suggest that formation of low-order Tau oligomers marks an early disease stage that is initiated by Tau phosphorylation at N-terminal sites. Formation of higher-order oligomers appears to require additional phosphorylation in the C-terminus of Tau. When used to assess Tau levels in human cerebrospinal fluid, the antibodies permitted to discriminate patients with Alzheimer disease or other dementia like vascular dementia, indicative that these antibodies hold promising diagnostic potential.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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