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Title: Tissue clearing for confocal imaging of native and bio-artificial skeletal muscle
Authors: Decroix, Lieselot
Van Muylder, Vicky
Desender, Linda
Sampaolesi, Maurilio
Thorrez, Lieven # ×
Issue Date: 20-Apr-2015
Publisher: Williams & Wilkins
Series Title: Biotechnic & Histochemistry vol:90 issue:6 pages:424-431
Abstract: Novel clearing techniques have revolutionized three-dimensional confocal imaging of the brain without the need for physical tissue sectioning. We evaluated three clearing methods, ScaleA2, Clear(T2), and 3DISCO for visualizing native and tissue engineered muscle by confocal microscopy. We found that Clear(T2) treatment improved the depth of visualization of immunohistochemical staining slightly, but did not improve depth of visualization of endogenous green fluorescent protein (GFP). ScaleA2 preserved endogenous GFP signal better and permitted significantly deeper GFP imaging, but it was incompatible with tropomyosin immunohistochemical staining. 3DISCO treatment preserved both endogenous GFP and immunohistochemical staining, and permitted significantly deeper imaging. Clearing time for the 3DISCO procedure is short compared to ScaleA2 and Clear(T2). We suggest that 3DISCO is the preferable clearing method for native and tissue engineered skeletal muscle tissue.
ISSN: 1052-0295
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Development and Regeneration, Campus Kulak Kortrijk
Department of Development and Regeneration - miscellaneous (+)
Cardiovascular Sciences, Campus Kulak Kortrijk
Department of Cardiovascular Sciences - miscellaneous
Stem Cell Biology and Embryology (+)
× corresponding author
# (joint) last author

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