Title: Piliation of Lactobacillus rhamnosus GG promotes adhesion, phagocytosis and cytokine modulation in macrophages
Authors: Vargas Garcia, Cynthia
Petrova, Mariya
Claes, I.
De Boeck, I.
Verhoeven, Tine
Dilissen, E.
von Ossowski, I.
Palva, A.
Bullens, Dominique
Vanderleyden, Jos
Lebeer, Sarah # ×
Issue Date: 2015
Publisher: American Society for Microbiology (ASM)
Series Title: Applied and Environmental Microbiology vol:81 pages:2050-2062
Abstract: Recently, spaCBA-encoded pili were identified on the cell surface of L. rhamnosus GG as key molecules for binding to human intestinal mucus and Caco-2 intestinal epithelial cells. Here, we investigated the role of the SpaCBA pilus of L. rhamnosus GG in the interaction with macrophages in vitro by comparing the wild-type with surface mutants. Our results show that SpaCBA pili play a significant role in the adhesion capacity to macrophages, and also promote bacterial uptake by these phagocytic cells. Interestingly, our data suggest that SpaCBA pili also mediate anti-inflammatory effects by induction of interleukin-10 (IL-10) mRNA and reduction of interleukin-6 (IL-6) mRNA in a murine Raw 264.7 macrophage cell line. These pili appear to mediate these effects indirectly by promoting close contact with the macrophages, facilitating other surface molecules to exert anti-inflammatory effects via yet unknown mechanisms. Blockage of complement receptor 3 (CR3), previously identified as a receptor for streptococcal pili, significantly decreased the uptake of pili-expressing strains in Raw 264.7 cells, while the expression of IL-10 and IL-6 mRNA by these macrophages was not affected by this blocking. On the other hand, blocking Toll-like receptor 2 (TLR2) significantly reduced the expression of IL-6 mRNA irrespective of the presence of pili.
ISSN: 0099-2240
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Laboratory of Pediatric Immunology
× corresponding author
# (joint) last author

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