Title: Strawberry pectin methylesterase (PME): Purification, characterization, thermal and high-pressure inactivation
Authors: Ly-Nguyen, B ×
Van Loey, Ann
Fachin, D
Verlent, Isabel
Duvetter, Thomas
Vu, ST
Smout, Chantal
Hendrickx, Marc #
Issue Date: 2002
Publisher: Amer chemical soc
Series Title: Biotechnology Progress vol:18 issue:6 pages:1447-1450
Abstract: Pectin methylesterase (PME) was extracted from strawberries (Fragaria ananassa, cv Elsanta) And purified by affinity chromatography on a. CNBr-Sepharose 4B-PME-inhibitor column. A single protein and PME activity peak was obtained; A biochemical characterization in terms of molecular mass, pI, and kinetic parameters of strawberry PME was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal and combined isothermal-isobaric inactivation of purified strawberry PME could be described by a fractional-conversion model. Purified strawberry PME is much more stable toward high-pressure treatments in comparison to those from oranges and bananas.
ISSN: 8756-7938
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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