Pectin methylesterase (PME) was extracted from strawberries (Fragaria ananassa, cv Elsanta) And purified by affinity chromatography on a. CNBr-Sepharose 4B-PME-inhibitor column. A single protein and PME activity peak was obtained; A biochemical characterization in terms of molecular mass, pI, and kinetic parameters of strawberry PME was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal and combined isothermal-isobaric inactivation of purified strawberry PME could be described by a fractional-conversion model. Purified strawberry PME is much more stable toward high-pressure treatments in comparison to those from oranges and bananas.