Title: Chemical changes of thermally sterilized broccoli puree during shelf-life: Investigation of the volatile fraction by fingerprinting-kinetics
Authors: Kebede, Biniam Tamiru
Grauwet, Tara
Magpusao, Johannes
Palmers, Stijn
Michiels, Chris #
Hendrickx, Marc
Van Loey, Ann # ×
Issue Date: 2015
Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science
Series Title: Food Research International vol:67 pages:264-271
Abstract: For the first time in literature, this study evaluates the potential of a “fingerprinting-kinetics” strategy to
investigate how the volatile fraction of thermally sterilized broccoli puree is affected by shelf-life. Samples were stored at four storage temperatures (20 °C, 28 °C, 35 °C and 42 °C). The volatile fraction was analyzed using headspace GC-MS fingerprinting as a function of time and temperature (kinetics). Fingerprinting enabled selection of sulfur-containing compounds (dimethyl sulfide, carbon disulfide, dimethyl pentasulfide, dimethyl trisulfide, bis-(methylthio)-phosphine and methyl (methylthio)methyl disulfide), nitriles (heptanonitrile, 5-methyl-hexanenitrile, hexanenitrile and 5-(methylthio)-pentanenitrile), fatty acid derivatives (octanal and 2-ethyl-1-hexanol), furanic compounds (2-ethylfuran) and Strecker aldehydes (3-methylbutanal) as volatiles significantly changing during shelf-life. In general, most of the volatiles decreased as a function of shelf-life.
Next, the suitability of the volatiles (selected by fingerprinting) as markers for accelerated shelf-life testing was investigated. Based on the applied kinetic modelling, 3-methylbutanal was identified as a potential marker.
ISSN: 0963-9969
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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