Title: HIV-2 integrase polymorphisms and longitudinal genotypic analysis of HIV-2 infected patients failing a raltegravir-containing regimen
Authors: Cavaco-Silva, Joana ×
Abecasis, Ana
Miranda, Ana Cláudia
Poças, José
Narciso, Jorge
Águas, Maria João
Maltez, Fernando
Almeida, Isabel
Germano, Isabel
Diniz, António
Gonçalves, Maria de Fátima
Gomes, Perpétua
Cunha, Celso
Camacho, Ricardo Jorge #
Portuguese HIV-2 Resistance Study Group #
Issue Date: 28-Mar-2014
Publisher: Public Library of Sciene
Series Title: PLoS One vol:9 issue:3 pages:e92747
Article number: 10.1371/journal.pone.0092747
Abstract: To characterize the HIV-2 integrase gene polymorphisms and the pathways to resistance of HIV-2 patients failing a raltegravir-containing regimen, we studied 63 integrase strand transfer inhibitors (INSTI)-naïve patients, and 10 heavily pretreated patients exhibiting virological failure while receiving a salvage raltegravir-containing regimen. All patients were infected by HIV-2 group A. 61.4% of the integrase residues were conserved, including the catalytic motif residues. No INSTI-major resistance mutations were detected in the virus population from naïve patients, but two amino acids that are secondary resistance mutations to INSTIs in HIV-1 were observed. The 10 raltegravir-experienced patients exhibited resistance mutations via three main genetic pathways: N155H, Q148R, and eventually E92Q - T97A. The 155 pathway was preferentially used (7/10 patients). Other mutations associated to raltegravir resistance in HIV-1 were also observed in our HIV-2 population (V151I and D232N), along with several novel mutations previously unreported. Data retrieved from this study should help build a more robust HIV-2-specific algorithm for the genotypic interpretation of raltegravir resistance, and contribute to improve the clinical monitoring of HIV-2-infected patients.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

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