Therapeutic Drug Monitoring vol:37 issue:4 pages:479-85
Determination of infliximab (IFX) serum concentrations has been used for treatment optimization of patients with inflammatory bowel disease (IBD). A wide range of ELISA assays exists to quantitate IFX. Most of these assays lack specificity and cross-react with other anti-tumor necrosis factor (TNF) agents. The ability of these IFX assays to detect IFX in complex with anti-drug antibodies is not known. The objective of our study was to develop an IFX-specific immunoassay to monitor IFX serum concentrations and to evaluate the impact of anti-drug antibodies on the assay performance.