Title: Biological, biochemical and physicochemical evidence for the existence of the polyadenylate - polyuridylate - poly 2'-fluoro-2'-deoxyuridylate triple-stranded complex
Authors: De Clercq, Erik ×
Janik, B
Sommer, R G #
Issue Date: Jul-1976
Publisher: Elsevier Pub. Co.
Series Title: Chemico-Biological Interactions vol:14 issue:1-2 pages:113-25
Abstract: The interferon-inducing activity of the double-stranded complex poly(A) - poly(U) in primary rabbit kidney cell cultures is reduced when the cells are treated with poly(dUfl) either 1 h before, simultaneously with, or 1 h after the exposure to the double-stranded complex. It has been demonstrated in experiments involving sensitivity to hydrolysis by RNAase, UV absorbance-mixing curves, and UV absorbance-temperature profiles that this phenomenon is due to the formation of the triple-stranded complex poly(A) - poly(U) - poly(dUfl). The latter complex seems to be the principal product of interactions in the following systems: poly(A) - poly(U) + poly(dUfl); poly(A) - poly(dUfl) + poly(U); and poly(A) + poly(U) + poly (dUfl).
ISSN: 0009-2797
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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