Revista Cubana de Ciencias Biologicas vol:2 issue:1 pages:22-31
Proteolytic enzymes such as collagenase, trypsin and pronase E are widely
used to acutely dissociate adult cardiomyocytes. There is some evidence that
enzyme treatment can alter ionic channels. The aim of the present investigation
was to compare the characteristics of the L-type Ca2+ current (ICaL) of
rat ventricular cardiomyocytes dissociated with two enzyme combinations:
collagenase + trypsin (C+T) and collagenase + pronase E (C+P). ICaL density
(pA/pF) was significantly lower (~ 2 pA/pF) in myocytes isolated with the
C+P combination. However, its inactivation time course was barely affected.
As well, the voltage dependency of ICaL kinetics was not affected by the C+P
treatment. Our results suggest that, compared to the C+T, treatment with the
C+P enzyme combination could decrease the number of functional (expressed)
channels in the sarcolemma.