Title: Unique stress response to the lactoperoxidase-thiocyanate enzyme system in Escherichia coli
Authors: Sermon, Jan ×
Vanoirbeek, K
De Spiegeleer, Philipp
Van Houdt, Rob
Aertsen, Abram
Michiels, Christiaan #
Issue Date: 2005
Publisher: Elsevier science bv
Series Title: Research in microbiology vol:156 issue:2 pages:225-232
Abstract: Using a differential fluorescence induction approach, we screened a promoter trap library constructed in a vector with a promoterless gfp gene for Escherichia coli MG1655 promoters that are induced upon challenge with the antimicrobial lactoperoxidase-thiocyanate enzyme system. None of the thirteen identified lactoperoxidase-inducible open reading frames was inducible by H2O2 or by the superoxide generator plumbagin. However, analysis of specific promoters of known stress genes showed some of these, including recA, dnaK and sodA, to be inducible by the lactoperoxidase-thiocyanate enzyme system. The results show that the lactoperoxidase-thiocyanate enzyme system elicits a distinct stress response different from but partly overlapping other oxidative stress responses. Several of the induced genes or pathways may be involved in bacterial defense against the toxic effects of the lactoperoxidase-thiocyanate enzyme system. (c) 2004 Elsevier SAS. All rights reserved.
ISSN: 0923-2508
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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